Application-Specific HPLC Systems

Analytical HPLC Systems for Food Analysis, Aflatoxins, Sugars, and Organic Acids

AZURA® Analytical HPLC Systems from Knauer Chromatography offer high-performance and user-value, with a tradition of ruggedness and engineering excellence

Our systems below have all been designed and optimized with specific applications in mind.

All AZURA® HPLC Systems are supplied with the appropriate pumps, injectors, detectors, and control capabilities. Details for each respective system are provided below.

Many additional HPLC modules and systems are available from iChrom Solutions. Please contact us for more information or a quotation.

AZURA® HPLC System for Food Analysis

Analytical HPLC system up to 700 bar with DAD detection

Our HPLC system for Food Analysis meets the demands of the most sophisticated analyses, with pressure capabilities up into the UHPLC range (700 bar).

With full gradient capability, an advanced autosampler, diode-array UV/Vis detection, and ClarityChrom software for control and data acquisition, this system can handle even the most complex applications in food analysis.

The AZURA® Food Analysis System includes:

  • 1 AZURA® Pump P 6.1L low pressure gradient pump with maximum pressure of 700 bar
  • 2 AZURA® Autosampler AS 6.1L for automated sample injection
  • 3 AZURA® Column thermostat CT 2.1 for column temperature control
  • 4 AZURA® DAD 2.1L diode array UV/Vis detector with 8-channel monitoring from 190-750 nm
  • 5 ClarityChrom® software for instrument control and data acquisition

The P 6.1L low pressure gradient pump operates with very precise valve control and features an internal degasser for efficient online degassing of the eluent. The mixing chamber of the pump is available in three different volumes depending on the application (100 μl is standard).

The AS 6.1L autosampler can inject up to 768 samples from microtiter plates or 108 standard 2 ml vial samples with a minimum injection volume of 0.1 μl.

The AZURA® CT 2.1 column thermostat holds up to 6 columns with a max. length of 300 mm and can maintain a constant temperature from 5 °C to 85 °C.

The AZURA® DAD 2.1L features a novel light path and flow cell architecture with improved handling and excellent performance. The frontal lamp and cell change ensure easy and secure maintenance. The temperature controlled optical bench minimizes signal drift; the wide flow cell selection allows the DAD 2.1L to be easily adapted to specific application needs.

MobileControl software provides AZURA® device control; monitor the status of several devices at once from a tablet or change instrument settings. MobileControl is the perfect complement to your chromatography data system. User Management capabilities ensure that system parameters can only be changed by authorized and logged-in users.

KNAUER ClarityChrom® is a powerful, yet easy-to-use PC-based chromatography data system for instrument control, data acquisition and data processing.

AZURA® HPLC System for Sensitive Aflatoxin Determination

With post-column photochemical derivatization and fluorescence detection

The AZURA® Aflatoxin analysis system is specially designed to determine Aflatoxin B1, B2, G1, and G2 in food and feed products such as peanuts, corn and cottonseed. It utilizes efficient post-column photochemical derivatization to improve fluorescence sensitivity for aflatoxins B1 and G1 drastically.

Aflatoxins are the best-known group of mycotoxins produced as secondary metabolites by fungi, mainly by Aspergillus flavus and Aspergillus parasiticus, and to a smaller extent by other fungal strains. (The designation “Aflatoxin” is derived from “Aspergillus flavus toxin”.)

iChrom Solutions

Aflatoxins are produced on crops in the field or during storage of agricultural products, especially under warm conditions and high humidity. Unfortunately, these toxins can persist long after the fungi have been killed, and thus contaminate foods. Most mycotoxins are highly stable and not destroyed during food processing or cooking.

As a hazard to public health, effective analytical methods for the identification and quantification of mycotoxins are required. Governmental regulatory laboratories and health protection agencies apply these methods on a large scale to control commercially marketed food products and animal feed.

Within the food processing industry the same methods are used for quality control on raw materials and products, to approve supply of these products to countries with mycotoxin regulation.

The AZURA® Aflatoxin System includes:

  • 1 AZURA® Pump P 6.1L low pressure gradient pump with maximum pressure of 700 bar
  • 2 AZURA® Autosampler AS 6.1L for automated sample injection
  • 3 AZURA® Column thermostat CT 2.1 for column temperature control
  • 4 AZURA® RF-20A Fluorescence detector, coupled to a photochemical post-column derivatization reactor for sensitive detection of aflatoxins
  • 5 Sample preparation kit consisting of SPE cartridges and method protocol
  • 6 Application description including method and analytical HPLC column
  • 7 ClarityChrom® software for instrument control and data acquisition; or AZURA® Mobile Control software for use with tablet PCs

AZURA® HPLC System for Sugar Analysis

HPLC system, ideally suited for the analysis of sugars, organic acids and alcohols

Carbohydrates – including simple sugars - alcohols, and organic acids are important in the manufacture of many foods, pharmaceuticals, and biofuels. This dedicated system is specially designed for simple and fast analyses of these compounds.

For example, in wine analysis it is desirable to measure all three of these compound classes; with this system it is possible to do so concurrently.

iChrom Solutions

The heart of the system is the superior AZURA® RID 2.1L refractive index detector with an outstanding linear range and sensitivity.

In addition, the AZURA® HPLC System for Sugar Analysis is economically priced to offer a low-cost option for these important applications.

The AZURA® Sugar Analysis System includes:

  • 1 AZURA® Pump P 6.1L isocratic pump with maximum pressure of 700 bar
  • 2 Manual valve for sample injection
  • 3 AZURA® CT 2.1 Column thermostat for column temperature control
  • 4 AZURA® RID 2.1L refractive index detector
  • 5 ClarityChrom® software for instrument control and data acquisition; or AZURA® Mobile Control software for use with tablet PCs