CHRONECT Symbiosis Advanced is the flagship CHRONECT Symbiosis model offered by iChrom, giving the most advanced integration with UHPLC analysis. As a result, CHRONECT Symbiosis Advanced provides the best analytical peak shape and the highest analyte sensitivity possible with online SPE/UHPLC. CHRONECT Symbiosis Advanced includes all of the modules in the CHRONECT Symbiosis Plus, but the Dual ACE (“Automated Cartridge Exchanger”) module is upgraded to handle online extractions up to 1,000 bar. This also requires special PEEK SPE cartridges of 1 mm diameter and very low dead volume (~8 µL) to give highly-efficient elution profiles, and thus taller analytical peaks.
Fundamental increased assay performance benefits of CHRONECT Symbiosis Advanced are the same as for our other CHRONECT Symbiosis systems, deriving from online SPE coupled to UHPLC.
These benefits include:
Because of its unique architecture, the CHRONECT Symbiosis Advanced offers the scientist three distinct operational capabilities (without replumbing!):
Any of these three options may serve as a front-end to multiple detectors (including MS and MS-MS), or even a fraction collector if desired.
Along with these powerful options, the 1,000-bar Dual ACE Module - and total of four UHPLC switching valves - adds all the benefits of two cartridges, including:
**With a Single ACE module, a sample is extracted via the single online cartridge, then the cartridge is eluted into the analytical column and UHPLC performed. With the Dual ACE module, the second cartridge allows for the next sample to be extracted during the previous sample’s analysis time. This can result in much shorter overall cycle times, and is ideal for laboratories requiring very high throughput. This process is intelligently and efficiently managed by the included CHRONOS CHRONECT Symbiosis software.
Finally, due to the 1,000-bar pressure compatibility of the ACE module – coupled with the narrow bore, 1 mm PEEK cartridges – CHRONECT Symbiosis Advanced online SPE delivers superior peak profiles to even those of the CHRONECT Symbiosis Plus system, especially for lower UHPLC analytical flow rates.
See below for more details on the CHRONECT Symbiosis Advanced system modules, features, and options.